DMOG (Dimethyloxallyl Glycine)

Catalogusnr.S7483 Batch:S748304

Afdrukken

Technische gegevens

Formule

C6H9NO5
Molecuulgewicht 175.14 CAS-nr. 89464-63-1
Oplosbaarheid (25°C)* In vitro DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol 35 mg/mL (199.84 mM)
In Vivo (Voeg oplosmiddelen afzonderlijk en in volgorde toe aan het product.)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml betekent licht oplosbaar of onoplosbaar.
* Houd er rekening mee dat Selleck de oplosbaarheid van alle verbindingen intern test en de werkelijke oplosbaarheid enigszins kan afwijken van gepubliceerde waarden. Dit is normaal en is te wijten aan lichte batch-tot-batch variaties.
* Verzending op kamertemperatuur (Stabiliteitstests tonen aan dat dit product zonder koelmaatregelen kan worden verzonden.)

Voorbereiden van stamoplossingen

Biologische activiteit

Beschrijving DMOG (Dimethyloxalylglycine) is een antagonist van de α-ketoglutaraat-cofactor en remmer van HIF prolylhydroxylase, wat leidt tot stabilisatie en accumulatie van HIF-1α-eiwit in de kern. DMOG verbetert autophagy.Oplossingen zijn onstabiel en moeten vers worden bereid.
Doelen
HIF prolyl hydroxylase
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. This compound reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC.

In Vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. Up-regulation of hypoxia-inducible factor-1α by this compound enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats.

Dichtheid 1.246 g/mL

Protocol (uit referentie)

Celassay:

[3]
  • Cellijnen

    VSMC cells

  • Concentraties

    ~100 μM

  • Incubatietijd

    72-96 hours

  • Methode

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.
Dierstudie:

[2]
  • Dierlijke modellen

    Mice (C57Bl6)

  • Doseringen

    8 mg in 0.5 ml saline

  • Toediening

    i.p.

Referenties

  • https://pubmed.ncbi.nlm.nih.gov/8002959/
  • https://pubmed.ncbi.nlm.nih.gov/15319416/
  • https://pubmed.ncbi.nlm.nih.gov/19304911/
  • https://pubmed.ncbi.nlm.nih.gov/24389644/

Klantproductvalidatie

Western blot was performed to analyze p65 signaling and the expression of Bcl2 and cleaved Caspase 3. GAPDH served as the loading control

Gegevens van [ , , Hepatology, 2018, 68(6):2359-2375 ]

Western blot analysis of the indicated proteins after the treatment with DMOG (K).

Gegevens van [ , , Theranostics, 2017, 7(13): 3293-3305 ]

Western blot analysis of the indicated proteins after the treatment with DMOG.

Gegevens van [ , , Theranostics, 2017, 7(13):3293-3305 ]

OCI‐LY7 cells were cultured with DMOG (1·5 mM) treatment for 96 h, bortezomib (5 nM), with combined treatment by bortezomib (5 nM) for another 5 h, after being pretreated with DMOG (1·5 mM) for 91 h. Apoptosis of AIDWT (upper) and AIDKO (lower) was assessed by detecting signals of the apoptosis maker annexin V and 7‐aminoactinomycin D (7‐AAD) (left); and statistical diagram of percentage for apoptosis cells (right). Bor = bortezomib.

Gegevens van [ , , Clin Exp Immunol, 2018, doi:10.1111/cei.13227 ]

Selleck's DMOG (Dimethyloxallyl Glycine) Is geciteerd door 57 Publicaties

Targeted inhibition of PARP-1 in pulmonary epithelial cells and macrophages via SPA-functionalized microparticles attenuates sepsis-induced lung injury [ Mater Today Bio, 2026, 37:102955] PubMed: 41809387
Qinggan Jianpi formula attenuates atherosclerosis by suppressing macrophage lactate transport to activate repair genes via H3K18 lactylation [ Chin Med, 2026, 21(1)121] PubMed: 42032694
PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblasts [ Nucleic Acids Res, 2025, 53(14)gkaf669] PubMed: 40705926
Lactylation modification of HIF-1α enhances its stability by blocking VHL recognition [ Cell Commun Signal, 2025, 23(1):364] PubMed: 40760493
Hydrogen sulfide alleviates high-salt-stimulated myocardial fibrosis through inhibiting hypoxia-inducible factor-1α [ Front Pharmacol, 2025, 16:1502269] PubMed: 40642013
Direct visualization of emergent metastatic features within an ex vivo model of the tumor microenvironment [ Life Sci Alliance, 2025, 8(1)e202403053] PubMed: 39419548
Distinct dynamics of parental 5-hydroxymethylcytosine during human preimplantation development regulate early lineage gene expression [ Nat Cell Biol, 2024, 10.1038/s41556-024-01475-y] PubMed: 39080410
FG-4592 protected haematopoietic system from ionising radiation in mice [ Immunology, 2024, 10.1111/imm.13797] PubMed: 38685744
An E3 ligase TRIM1 promotes colorectal cancer progression via K63-linked ubiquitination and activation of HIF1α [ Oncogenesis, 2024, 13(1):16] PubMed: 38769340
Proliferative Diabetic Retinopathy Microenvironment Drives Microglial Polarization and Promotes Angiogenesis and Fibrosis via Cyclooxygenase-2/Prostaglandin E2 Signaling [ Int J Mol Sci, 2024, 25(20)11307] PubMed: 39457089

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NIET VOOR HUMANE, VETERINAIRE DIAGNOSTISCHE OF THERAPEUTISCHE DOELEINDEN.